Relación entre el perfil inflamatorio y la expresión de los factores de transcripción PPAR en leucocitos de niños con obesidad.

Abstract

Obesity is a pathology associated with abnormal fat deposition in adipocytes, which causes inflammation and insulin resistance, the risk of diabetes. This inflammatory process is associated with the activity of peroxisome proliferator-activated receptors (PPAR) that have an anti-inflammatory function. However, the role of these receptors in leukocytes and their relationship with inflammation in childhood obesity is unknown. Blood samples from a group of healthy and obese children between 7 and 17 years of age will be used for subsequent analysis of gene expression of the PPAR isotypes present in their leukocytes by qPCR. Serum levels of cytokines, chemokines, and adipokines were included by flow cytometry. An increase in interleukin-8, Interleukin-6, tumor necrosis factor-α, interferon-γ, monocyte chemotactic protein-1 and adipsin was found in obesity, while the increase in adiponectin was controlled in the group. Thus, a relationship between childhood obesity and a pro-inflammatory profile of serum cytokines, adipokines, and chemokines is evident. Likewise, PPAR-α was expressed mainly in the obese group and presented a negative correlation with TNF-α levels, indicating a relationship between PPAR-α expression and the inflammatory process of children with obesity.