Inhibición de la lipasa porcina por orlistat: un ejercicio para entender la cinética enzimática, el mecanismo de acción del fármaco y la estructura enzimática

Abstract

Inhibition of pancreatic lipase with Orlistat was used to show the correlation between experimental procedures and computational simulations. Both methods are important to understand the kinetics and the inhibition mechanism, and they complement each other in terms of the scope of the results obtained.The enzymatic activity of porcine pancreatic lipase was evaluated by determining kinetic parameters, such as the Michaelis constant- Menten and maximum speed, values corresponding to 61mM and 74µM / min, respectively. Orlistat inhibition tests were performed to obtain Lineweaver-Burk and Dixon graphs, complementary tools with which competitive inhibition was determined. In addition, a molecular coupling was included, to visualize the enzyme-Orlistat binding, along with a lipase sequence analysis to compare the structure of the enzyme in different organisms.