1. Universidad Antonio Nariño
  2. Trabajos de grado
  3. Pregrado
  4. Bioquímica
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dc.contributor.advisorGarcía Contreras, Germán Antonio-
dc.creatorSilva Yate, Edith Vanessa-
dc.date.accessioned2021-02-22T14:21:35Z-
dc.date.available2021-02-22T14:21:35Z-
dc.date.created2020-06-03-
dc.identifier.urihttp://repositorio.uan.edu.co/handle/123456789/1599-
dc.description.abstractCancer diagnostic methods are currently based on glycosylated biomarkers such as Tn antigen and can be detected by lectin-based technologies such as biosensors. However, there is a limitation due to the lack of commercial availability of lectins that recognize these structures, in addition to the fact that low stability may occur in biosensors due to the layers of proteins associated with them. In the present work we analyzed peanut lectin (Arachis Hypogaea), together with other lectins from legumes belonging to the Fabaceae family such as Glycine max (PDB 4D69), Wisteria Floribunda (PDB 5KXB), Psophocarpus Tetragonolobus (PDB 2D3S), and Isolectin B4 Vicia Villosa (1N47); where, based on their characteristics, peptides were designed and modeled and analyzed by means of molecular docking tests. Twelve candidate peptides were obtained for the library, eleven of which come from the structural segmentation of our protein of interest Arachis Hypogaea and one obtained from the comparisons and alignments of all the fabaceous proteins. The peptides analyzed by molecular docking were considered stable, of adequate length, with energy properties similar to peanut lectin when coupled with the antigen Tn, being considered excellent candidates to be later synthesized and analyzed for possible cancer diagnostic methods.es_ES
dc.description.tableofcontentsLos métodos de diagnóstico del cáncer actualmente se basan en biomarcadores que se encuentran glucosilados como lo es el antígeno Tn y pueden ser detectados por tecnologías basadas en lectinas como son los biosensores. Sin embargo, hay una limitación por la falta de disponibilidad comercial de lectinas que reconocen estas estructuras, además de que puede presentarse baja estabilidad en los biosensores por las capas de proteínas asociadas a este. En el presente se trabajo se analizó la lectina de maní (Arachis Hypogaea), en conjunto de otras lectinas de leguminosas pertenecientes la familia Fabaceae como Glycine max (PDB 4D69), Wisteria Floribunda (PDB 5KXB), Psophocarpus Tetragonolobus (PDB 2D3S), e Isolectina B4 Vicia Villosa (1N47); en donde basándose en sus características se diseñaron y modelaron péptidos los cuales fueron analizados por medio de pruebas de docking molecular. Se obtuvieron doce péptidos candidatos para la librería los cuales, once provienen de la segmentación estructural de nuestra proteína de interés Arachis Hypogaea y uno obtenido en las comparaciones y alineamientos de todas las proteínas fabáceas. Los péptidos analizados mediante docking molecular se consideraron estables, de longitud adecuada, con propiedades energéticas similares a la lectina de maní cuando se encuentra acoplado con el antígeno Tn, considerándose así unos excelentes candidatos para que posteriormente puedan ser sintetizados y analizados para ser posibles métodos de diagnóstico de cáncer.es_ES
dc.language.isospaes_ES
dc.publisherUniversidad Antonio Nariñoes_ES
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Estados Unidos de América*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/us/*
dc.subjectAntígeno Tnes_ES
dc.subjectPNAes_ES
dc.subjectMucinases_ES
dc.subjectGlicosilaciónes_ES
dc.subjectCánceres_ES
dc.subjectAcetilgalactosaminaes_ES
dc.titleDiseño de secuencias peptídicas derivadas de la lectina de arachis hypogaea para el reconocimiento del antígeno Tnes_ES
dc.publisher.programBioquímicaes_ES
dc.rights.accesRightsopenAccesses_ES
dc.subject.keywordTn antigenes_ES
dc.subject.keywordPNAes_ES
dc.subject.keywordMucinses_ES
dc.subject.keywordGlycosylationes_ES
dc.subject.keywordCanceres_ES
dc.subject.keywordAcetylgalactosaminees_ES
dc.type.spaTrabajo de grado (Pregrado y/o Especialización)es_ES
dc.type.hasVersioninfo:eu-repo/semantics/acceptedVersiones_ES
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dc.description.degreenameBioquímico(a)es_ES
dc.description.degreelevelPregradoes_ES
dc.publisher.facultyFacultad de Cienciases_ES
dc.description.notesPresenciales_ES
dc.publisher.campusBogotá - Circunvalar-
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