Degradación del insecticida clorpirifos por Pseudomona aeruginosa, rizobacteria aislada de cultivo de papa (Solanum tuberosum)

Abstract

The insecticide chlorpyrifos is used on a variety of agricultural crops around the world. and its large-scale application has caused environmental contamination. In this work, the degradation rate of chlorpyrifos by the rhizobacterium Pseudomonas aeruginosa (TN50) from potato (Solanum tuberosum) crop in minimal salt medium was determined. Genes and enzymes were detected in the genome of TN50 that may be associated with chlorpyrifos degradation and suggest that the bacterium may degrade the insecticide as a source of phosphorus and carbon. The chromatographic and liquid-liquid extraction technique for the monitoring of chlorpyrifos and its main degradation metabolite 3,5,6 trichloro-2-pyridinol was validated and the capacity of TN50 to use the insecticide as a sole carbon source was established. A 17.43% depletion of chlorpyrifos by P. aeruginosa was determined with glucose as an external carbon source and with prior metabolic activation in medium with glucose and chlorpyrifos, indicating that TN50 does not have the capacity to use the insecticide as a sole carbon source but can degrade the compound as an external nutrient source. Additional degradation studies could determine the potential of TN50 for bioremediation of this type of pollutants.