Evaluación de la unión de un péptido marcador de neuroinflamación con diferentes proteínas blanco y su conjugación con nanopartículas superparamagnéticas

Abstract

Neuroinflammation is characterized by presenting alterations in the blood-brain barrier, and activation of glial cells, leading to brain tissue alterations generating a pathology, this has led to the need to improve the magnetic resonance imaging technique that allows understanding the development and progression of alterations of the blood-brain barrier in real time, under neuroinflammatory conditions. As an alternative to this solution, it is proposed to establish the binding of a neuroinflammation marker peptide with its target protein and its conjugation with superparamagnetic nanoparticles. The results show that the magnetic nanoparticles have a spherical shape with a size of 54 ± 10 nm, the chemical composition Fe @ SiO2 and a magnetic hysteresis curve indicating a superparamagnetic behavior; The evaluation of the binding of peptide-88 with its possible receptor protein showed a greater binding between peptide-88 with laminin β1, likewise the binding of peptide-88 with the nanoparticles was studied, demonstrating a greater binding unlike the target. The present study showed that by establishing the functionalization of superparamagnetic nanoparticles conjugated to a neuroinflammation biomarker peptide, they can be used for early diagnosis and real-time evolution of the mechanisms triggered under neuroinflammatory conditions.