Proliferación celular en andamios de fibrina, agarosa y fibrina-agarosa: revisión narrativa

Abstract

Introduction: Scaffolds must be designed to replicate the native tissue architecture in vitro, so that cells adhere, spread, proliferate, differentiate and mature, in a similar way to what they do in vivo. The objective of this review was to analyze the scientific evidence available on cell proliferation in scaffolds based on fibrin, agarose and fibrin-agarose mixture biomaterials. Materials and methods: Narrative review, bibliographic search of the last 5 years published in Medline-PubMed and Web of Science. during the period of August and September 2020. Inclusion criteria: 1). Experimental studies in vitro, in vivo, ex vivo, 2) Studies that will evaluate cell proliferation in fibrin, agarose and fibrin-agarose mixture scaffolds, 3). Preclinical trials, 4). Bibliographic reviews, 5). Articles published in English and Spanish, 6). Documents published between 2016 and 2020. Thematic analysis was carried out by topics in each scaffold: cell types, types of cell proliferation assays, cell proliferation evaluation times and cell proliferation results. Results: 40 articles, 21 for fibrin, 9 for agarose, 10 for fibrin-agarose. The cells most used in the studies of the three scaffolds were mesenchymal stem cells of various origins. The most widely used types of assay were DNA quantification, the Ki67 proliferation marker and the cellular proliferation nuclear antigen (PCNA). The most used cell proliferation evaluation time period was 1 to 7 days. The qualitative proliferation results were more prevalent than the quantitative ones, finding great diversity in the reports. Conclusions: It is reported that the three scaffolds object of the review in general have the ability to promote cell proliferation.